Present study has been conducted to evaluate antiurolithiatic activity of 3 different plants i.e. Nardostachys jatamansi rhizome, Achyranthes aspera L whole plant and Trachyspermum ami fruit and its comparison with formulation developed from that plants. Antiurolithiatic activities of all hydroalcoholic extracts were assessed in-vitro by measuring level of undissolved calcium in each group and in-vivo by analyzing different urine parameters i.e. urine volume, pH, calcium level, phosphate level, creatinine level, oxalate level, magnesium level, uric acid level as well as serum parameters i.e. serum creatinine, blood urea nitrogen, uric acid against ethylene glycol induced nephrolithiasis in male rats. % Dissolution of calcium oxalate in hydroalcoholic extracts of all the 3 plants is higher as compare to negative control. Hydroalcoholic extract of churna shows synergistic effect and having capacity to dissolve more amount of calcium oxalate as compare to individual plants extracts. Cystone was used as standard drug to compare activities of test samples. In-vivo antiurolithiatic activity represent that hydroalcoholic extracts of all the 3 plants decreases levels of all above urine and serum parameters which was increased significantly in rats treated with ethylene glycol only. Synergistic effect observed in rats treated with hydroalcoholic extract of Churna. The study was also supported by histopathology of kidney sections. Present study reveals that the hydroalcoholic extracts of Nardostachys jatamansi rhizome, Achyranthes aspera L. whole plant and Trachyspermum ami fruit could afford a significant antilithiatic activity and hydroalcoholic extract of Churna having better antilithiatic activity as compared to individual plants.
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